[Validation of a questionnaire of lycopene frequency intake]. / Validación de un cuestionario de frecuencia de consumo de licopeno.

INTRODUCTION: An inverse relationship between some chronic degenerative diseases and plasma lycopene levels has been demonstrated. Dietary intake questionnaires are one of the current methods most used to ascertain dietary patterns and explore their association with the disease risk. The main drawback of their use is the need for previous validation. The purpose of this study was to validate a frequency questionnaire in order to assess the intake of licopene, in the population of the Canary Islands. METHODS: A food intake frequency questionnaire was designed and administered to 70 patients of the Plastic Surgery Service of the Hospital Universitario Insular de Gran Canaria. Estimated lycopene intake from the food intake frequency questionnaire was examined in relation to plasma lycopene levels, measured by HPLC. RESULTS: The Spearman correlation coefficient between estimated lycopene intake and plasma levels was 0,421 and the validity of the questionnaire was demonstrated. Furthermore, an association between obesity and some pathologies with plasma lycopene levels was observed, although not statistically significant. CONCLUSIONS: The food intake frequency questionnaire is valid and it could be useful in epidemiological studies in the population of the Canary Islands.

Influencia del procedimiento culinario sobre la biodisponibilidad del licopeno en el tomate / Influence of cooking procedure on the bioavailability of lycopene in tomatoes

Fundamento y objetivo: Estudio de intervención para determinar la influencia del procedimiento culinario del tomate sobre la biodisponibilidad del licopeno. Población y método: El estudio fue realizado sobre una muestra constituida por 15 individuos que participaron de forma voluntaria. La determinación de los niveles de licopeno en las muestras sanguíneas de los pacientes se realizó mediante la técnica de HPLC. Resultados: La ingestión del producto fresco triturado no modifica, en forma significativa, los niveles de licopeno en sangre de los sujetos de la muestra. Tanto la trituración con aceite de oliva como el tratamiento térmico del tomate en combinación con aceite de oliva incrementaron, significativamente, los niveles de licopeno plasmático. Conclusiones: La biodisponibilidad del licopeno depende del procesado del producto. La asociación con ácidos grasos así como que el tratamiento térmico del tomate aumentan su biodisponibilidad (AU)

Background and objective: The aim of this study was to evaluate the influence of raw and processed tomato consumption on plasma lycopene concentration in healthy volunteers. A cross-over dietary intervention study was employed. Patients and methods: Fifteen healthy subjects were included in the study. Plasma lycopene concentration was assayed by HPLC. Results: Raw crushed tomato consumption did not significantly influence plasma lycopene concentration. Consumption of raw crushed tomato with olive oil and cooked tomatoes with olive oil, significantly increased blood lycopene levels (AU)

Validación de un cuestionario de frecuencia de consumo de licopeno / Validation of a questionnaire of lycopene frequency intake

Introducción: Ha sido demostrada una relación inversa entre los niveles de licopeno en el organismo y la aparición de algunas enfermedades crónico-degenerativas. Los cuestionarios de frecuencia de consumo de alimentos son una de las herramientas más utilizadas en los estudios epidemiológicos para estimación de la ingestión de nutrientes. La necesidad de validación previa a su utilización constituye su principal inconveniente. La validación de un cuestionario de frecuencia de consumo de licopeno, adaptado a la población de las Islas Canarias, es el objetivo del presente trabajo. Material y métodos: Se diseñó un cuestionario semi-cuantitativo de frecuencia de consumo de alimentos, en el que fueron incluidos alimentos con elevado contenido en licopeno. El cuestionario fue aplicado a un grupo de 70 pacientes del Servicio de Cirugía Plástica del Hospital Universitario Insular de Gran Canaria. Se utilizó como patrón de oro, para su validación, los niveles séricos de licopeno de la población encuestada, determinados mediante la técnica de HPLC. Resultados: Se encontró una correlación directa entre la ingesta de alimento y los niveles en sangre del carotenoide, siendo el coeficiente de correlación de Spearman estimado 0,421. Se observó una asociación de los niveles de licopeno en sangre con la obesidad y algunas patologías, aunque no fue estadísticamente significativa. Conclusiones: La correlación obtenida entre la ingesta de alimentos conteniendo licopeno y los niveles séricos del micronutriente medidos, indica la validez del cuestionario y permite su utilización en estudios epidemiológicos (AU)

Introduction: An inverse relationship between some chronic degenerative diseases and plasma lycopene levels has been demonstrated. Dietary intake questionnaires are one of the current methods most used to ascertain dietary patterns and explore their association with the disease risk. The main drawback of their use is the need for previous validation. The purpose of this study was to validate a frequency questionnaire in order to assess the intake of licopene, in the population of the Canary Islands. Methods: A food intake frequency questionnaire was designed and administered to 70 patients of the Plastic Surgery Service of the Hospital Universitario Insular de Gran Canaria. Estimated lycopene intake from the food intake frequency questionnaire was examined in relation to plasma lycopene levels, measured by HPLC. Results: The Spearman correlation coefficient between estimated lycopene intake and plasma levels was 0,421 and the validity of the questionnaire was demonstrated. Furthermore, an association between obesity and some pathologies with plasma lycopene levels was observed, although not statistically significant. Conclusions: The food intake frequency questionnaire is valid and it could be useful in epidemiological studies in the population of the Canary Islands (>U)

[Influence of cooking procedure on the bioavailability of lycopene in tomatoes]. / Influencia del procedimiento culinario sobre la biodisponibilidad del licopeno en el tomate.

BACKGROUND AND OBJECTIVE: The aim of this study was to evaluate the influence of raw and processed tomato consumption on plasma lycopene concentration in healthy volunteers. A cross-over dietary intervention study was employed. PATIENTS AND METHODS: Fifteen healthy subjects were included in the study. Plasma lycopene concentration was assayed by HPLC. RESULTS: Raw crushed tomato consumption did not significantly influence plasma lycopene concentration. Consumption of raw crushed tomato with olive oil and cooked tomatoes with olive oil, significantly increased blood lycopene levels.

Estabilidad y compatibilidad de la mezcla de tramadol, ketorolaco, metoclopramida y ranitidina en una solución para perfusión intravenosa / Stability and compatibility of the mixture of tramadol, ketorolac, metoclopramide and ranitidine in a solution for intravenous perfusion

Objetivo Establecer si una mezcla para perfusión intravenosa que contiene tramadol (5mg/ml), ranitidina (1,5mg/ml), ketorolaco (1,5mg/ml) y metoclopramida (0,5mg/ml) en cloruro sódico al 0,9% es compatible y estable a temperatura ambiente durante un periodo de 48h.MétodosSe realizó un estudio de estabilidad de la mezcla mediante la técnica de cromatografía líquida de alta presión, comprobando visualmente, de forma paralela, los posibles cambios de color, la aparición de precipitado o la separación de fases indicativos de incompatibilidad entre los componentes. Resultados Los datos de la cromatografía mostraron al final del ensayo una concentración media para la metoclopramida comprendida entre el 100–105% de la inicial, mientras que para el tramadol, el ketorolaco y la ranitidina, las concentraciones obtenidas se encontraron entre el 99 y el 102% de las de partida. No hubo evidencia de incompatibilidad entre los fármacos a lo largo del tiempo de estudio. Conclusiones La combinación es estable en solución y sus componentes son física y químicamente compatibles en las concentraciones utilizadas en el estudio durante al menos 48h a temperatura ambiente (AU)

Objective To determine whether a mixture for intravenous perfusion containing tramadol (5mg/ml), ranitidine (1.5mg/ml), ketorolac (1.5mg/ml) and metoclopramide (0.5mg/ml) in a 0.9% sodium chlorides solution is compatible and stable at room temperature during a 48-hour period. Methods We tested the mixture for stability using the HPLC technique (high performance liquid chromatography), with parallel visual assessments of any changes in colour, appearance of precipitate or phase separation indicating incompatibilities between the components. Results At the end of the trial, chromatography data showed a mean metoclopramide concentration between 100% and 105% of the initial level, while concentrations of tramadol, ketorolac and ranitidine were between 99% and 102% of initial levels. There was no evidence of incompatibility between the drugs at any time during the study period. Conclusions The combination is stable as a solution and its components are physically and chemically compatible in the concentrations used in the study, during at least 48 hours at room temperature (AU)

Stability and compatibility of the mixture of tramadol, ketorolac, metoclopramide and ranitidine in a solution for intravenous perfusion.

OBJECTIVE: To determine whether a mixture for intravenous perfusion containing tramadol (5 mg/ml), ranitidine (1.5 mg/ml), ketorolac (1.5 mg/ml) and metoclopramide (0.5 mg/ml) in a 0.9% sodium chlorides solution is compatible and stable at room temperature during a 48-hour period. METHODS: We tested the mixture for stability using the HPLC technique (high performance liquid chromatography), with parallel visual assessments of any changes in colour, appearance of precipitate or phase separation indicating incompatibilities between the components. RESULTS: At the end of the trial, chromatography data showed a mean metoclopramide concentration between 100% and 105% of the initial level, while concentrations of tramadol, ketorolac and ranitidine were between 99% and 102% of initial levels. There was no evidence of incompatibility between the drugs at any time during the study period. CONCLUSIONS: The combination is stable as a solution and its components are physically and chemically compatible in the concentrations used in the study, during at least 48 hours at room temperature.